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ReadSim- A Simulator for Sanger and 454 Sequencing
New sequencing technology based on pyro-sequencing (as recently
introduced
by 454 Life Sciences) promises to be ideally suited for metagenomic
projects that seek to determine the diversity of organisms in a given
environment. To develop and calibrate software to process the arising
data, a sequencing simulator is required that samples reads from a
given known target sequence under an error model that reflects the
performance of the sequencing technology. Additionally, to simplify the
comparison of pyro-sequencing and Sanger sequencing, it is desirable
that both approaches can be simulated by a single program.
Here we provide a sequencing simulator ReadSim that simulates the
sequencing of reads both for Sanger and pyro-sequencing, optionally
involving mate-pairs.
Paper
A paper on ReadSim has been submitted:
R. Schmid, S.C. Schuster, M.A. Steel and D.H. Huson, ReadSim- A simulator for Sanger and 454
sequencing, 2006.
Use of the program is free for academic purposes at an academic
institute. For all other uses, please contact the authors.
Download
ReadSim_macos_0_10.dmg
ReadSim_windows_0_10.exe
ReadSim_unix_0_10.sh
Manual
To find out more about the program, take a look at the user manual.
Authors
The program was written by Ramona Schmid and Daniel Huson
Internal download page.
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Software